LC-MSMS | Direct injection method was used to determine glyphosate and glyphosate in rice

Glyphosate is a non-selective, non-residue herbicide; glufosinate-ammonium can effectively remove most glyphosate-resistant weeds, and is favored by all walks of life because of its environmental protection and easy degradation. With the widespread use of these substances, problems such as livestock poisoning and environmental pollution caused by glyphosate and glufosinate are increasing year by year. Rice is the most commonly used food raw material in daily life. The national standard "GB 2763-2021" stipulates that the maximum residue levels (MRL) of glyphosate and glufosinate in rice are 0.1 mg/kg and 0.9 mg/kg respectively .

Glyphosate is a highly polar compound, insoluble in most organic solvents, and has poor volatility. These characteristics greatly increase the difficulty of analysis. The use of gas chromatography and liquid chromatography, including liquid chromatography mass spectrometry, will encounter difficulties such as peak tailing . Therefore, the detection of such substances generally requires derivatization before analysis. However, the derivatization process is often cumbersome and time-consuming. hour.

Based on the analytical method for highly polar pesticide residues in food (Quppe) developed by the European Union, this application center uses hydrophilic interaction chromatography column-tandem mass spectrometry (HILIC-MS/MS) technology to establish the presence of glyphosate and glyphosate in rice. Glufosinate ammonium detection method. The experiment examined the linearity, sensitivity, precision and accuracy of the method. Using a highly sensitive and anti-pollution LC-MS/MS system, sensitive and accurate quantitative detection of glyphosate and glufosinate-ammonium residues in rice can be carried out.